CV
Education
- PhD. Entomology (2016). University of Georgia, Advisor: Dr. Michael J. Adang.
- M.Phil. Biochemistry (2011). University of Sussex, United Kingdom, Advisor: Dr. Neil Crickmore.
- M.Sc. Genetic Manipulation and Molecular Cell Biology (2007). University of Sussex, Advisor: Dr. Neil Crickmore.
Work experience
- August 2016- Present: Post-Doctoral Associate
University of Florida
Project 1: Engineer pesticidal protein to improve its efficacy against Lepidopteran insect pest (Industry funded).
- Developed protocol for in vivo bio-panning assay against insects using phage display library.
- Developed a pipeline to analyze Next Generation Sequencing in vivo bio-panning assay data.
- Standardized UV-crosslink pull down assay for identification of binding partners of gut binding peptides.
- Protein modelling and modification.
Standardized MicroScale Thermophoresis (MST) assay for studying protein-protein interaction.
- Project 2: Working as a team member for development of Bacterial Pesticidal Protein Resource Center.
- https://bpprc.org/ (under development)
Providing new nomenclature system for assigning names to bacterial pesticidal protein based on protein domain it possesses.
- PI: Dr. Bryony Bonning
Research Techniques
- Bottom-up proteomics: Sample preparation, Cell-surface protein enrichment, In-gel and In-solution digestion.
Protein analytical techniques such as SDS-PAGE, Native PAGE, Western blot, Dot blot, ELISA, ion exchange, affinity and Size exclusion chromatography.
- Peptide phage display library screening.
- Baculovirus expression of recombinant protein in insect cell lines.
- Confocal Microscopy.
- Molecular biology techniques: Gene cloning, RNA and DNA extraction, PCR, cDNA synthesis.
Bioinformatics and Programming Skills
- NGS RNA sequencing data analysis: reference genome based ((TopHat and Cufflinks) and de novo (Trinity), DESeq and Bioconductor.
Single Nucleotide Variant analysis: VarScan, SnpEff.
- LC-MS/MS data analysis: Mascot, ProteoIQ, Trans Proteomic Pipeline and Proteome Discoverer.
Next-generation sequencing of Phage display library and data analysis.
- R, Tidyverse (R package).
- Protein Modelling: PyMOL